MTase-Glo™ Methyltransferase Assay
Measure Methyltransferase Activity Using a Universal Bioluminescent Assay
- Simple add-and-read format
- Use with any methyltransferase
- No need to modify substrates
Catalog Number:
Size
Catalog Number: V7601
Catalog Number: V7602
Universal Assay
An Overview of the MTase-Glo™ Methyltransferase Assay
Measure the Activity of a Wide Array of Methyltransferases
Whether you are interested in protein, DNA, RNA or small molecule methyltransferases, the MTase-Glo™ Methyltransferase Assay can measure the activity of them all based on the reaction product S-adenosyl homocysteine (SAH). After the methyltransferase reaction is complete, just add the MTase-Glo™ Reagent and MTase-Glo™ Detection Solution to convert SAH to ATP and measure the light from the luciferase reaction.
Luminescence is measured using a plate-reading luminometer and can be correlated to SAH concentration using an SAH standard curve. The half-life of the luminescent signal is greater than 4 hours. This extended signal half-life eliminates the need for luminometers with injectors and allows batch-mode processing of multiple plates.
Determine the Optimal Enzyme Concentration
Titration of Methyltransferases Using the MTase-Glo™ Assay
Sensitive Detection
Luminescent Assay Offers Low Background and Wide Dynamic Range
The MTase-Glo™ Methyltransferase Assay produces excellent signal-to-noise ratios at low enzyme concentrations. Use the assay with a broad range of substrates, with no limitations on using high substrate concentrations or substrate type (short versus long peptides), to generate kinetic data and determine the mechanism of action of various methyltransferase modulators. There is also no need to modify substrates, which can lead to kinetic artifacts. Optimized for screening applications, the MTase-Glo™ Assay is not susceptible to false hits from fluorescence interference.
High-concentration SAH (up to 10µM)
Low-concentration SAH (up to 1µM)
Measure Methyltransferase Activity Using a Universal Bioluminescent Assay
The MTase-Glo™ Methyltransferase Assay is a novel, bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in many applications, including high-throughput screening.
Monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases.
Used successfully for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
SAM, 1mM |
A120B | 1 × 200μl |
SAH, 15µM |
A233B | 1 × 300μl |
MTase-Glo™ Reagent, 10X |
V760A | 1 × 1ml |
MTase-Glo™ Detection Solution |
V761A | 1 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
SAM, 1mM |
A120C | 1 × 1ml |
SAH, 15µM |
A233B | 1 × 300μl |
MTase-Glo™ Reagent, 10X |
V760B | 1 × 5ml |
MTase-Glo™ Detection Solution |
V761B | 1 × 50ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
