Bacterial Strain JM109(DE3)
For High-Level Gene Expression from the T7 Promoter
- Contains an IPTG-inducible gene for T7 RNA polymerase
- Requires a ribosomal binding site with cloned sequence
- Lacks blue/white cloning selection
Catalog Number:
Size
Catalog Number: P9801
Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection.
Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F´, traD36, proAB, lacIqZΔM15], lDE3.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Bacterial Strain JM109(DE3) |
P980A | 1 × 500μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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