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Express Regulatory Proteins in Mammalian Cells
The CCNB1 Expression Vector contains the coding region of the human CCNB1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The Expression Vector and kinase-NanoLuc® Fusion Vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein Expression Vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.
For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein Expression Vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.
Vector Details:
- Gene name: CCNB1
- Sequence
- Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent Format
About Kinase Fusion Vector Names
The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET® TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).
NanoBRET® Target Engagement (TE) Intracellular Kinase Assays
The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
CCNB1 Expression Vector |
NV260A | 3 × 20μg | 1μg/μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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