Promega Corporation

Proteasome-Glo™ Assay Systems Technical Bulletin

Share

Literature # TB349

The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays that measure the three proteolytic activities associated with the proteasome (each of these three assays is available separately). The Proteasome-Glo™ 3-Substrate System provides three separate assays that differ in their ability to detect different protease activities based on their substrate components. The luminogenic substrates provided for the chymotrypsin-like, trypsin-like, and caspase-like activities are Suc-LLVY-aminoluciferin, Z-LRR-aminoluciferin, and Z-nLPnLD-aminoluciferin, respectively. Each substrate is added to a buffer system optimized for proteasome activity and luciferase activity to make a Proteasome-Glo™ Reagent for a particular catalytic activity. The individual Proteasome-Glo™ Reagent is added to test samples in an "add-mix-measure" format, resulting in proteasome-induced cleavage of the particular substrate. Substrate cleavage generates a "glow-type" luminescent signal produced by the luciferase reaction. In this homogeneous coupled-enzyme format, the signal is proportional to proteasome activity.

Printed in USA. Revised 9/11.

 

PDF Icon Complete Protocol PDF (936kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Spring adventures

spring500x300