Literature # 9PIM991
TSAP Thermosensitive Alkaline Phosphatase catalyzes the removal of 5′ phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3′ overhangs, 5′ overhangs and blunt ends. It is also useful for preparing DNA for 5′ end-labeling by removing existing phosphate groups from the 5′ end.
Printed in USA. Revised 3/12.