The genetic, molecular and morphological aspects of apoptosis currently are under intensive study because of the important role apoptosis has in development and homeostasis, as well as in several disease states. Promega's DeadEnd™ Colorimetric Apoptosis Detection System labels fragmented DNA, one of the biochemical hallmarks of apoptosis. This colorimetric system is ideal for labeling apoptotic nuclei in tissue sections and cultured cells, while allowing for concurrent morphological assessment. In this report, the DeadEnd™ Apoptosis System is demonstrated in situ using cellular and animal models of apoptosis and in multiple pathology tissue sections.
Promega Notes 69, 2.
Martha O'Brien1, Angela Okragly1, Tom Warner2 Ron Kalil2 and Guus Wolswijk3
1Promega; 2University of Wisconsin Medical School; 3Netherlands Institute for Brain Research