Mammalian Expression Vectors

HaloTag® Fusion Vectors

Choose from N- or C-terminal HaloTag fusion Vectors that allow variable expression of proteins from the full-length CMV promoter or CMV deletion mutants. The HaloTag allows imaging or purification of expressed fusion proteins.

• N-Terminal HaloTag^® Vectors
• C-Terminal HaloTag^® Vectors

Mammalian Two-Hybrid System Vectors

Part of the CheckMate™ Flexi® System, the pFN10A(ACT) and pFN11A(BIND) Vectors are used to confirm, validate and study suspected interactions between two proteins or domains.

• Protocol
• Product Details + Ordering

pTARGET™ Mammalian Expression Vector

Easily clone and express PCR products in mammalian cells. Contains CMV promoter region to promote constitutive expression of cloned DNA. Neomycin resistance gene for selection of transfected cells.

• Product Details + Ordering

Regulated Mammalian Expression

Rapidly turn expression on or off. The pF12A and pF12K Flexi® Vectors, and the pReg neo Vector allow dose-response induction of protein expression.

• Product Details + Ordering

Here we introduce the Flexi Vector Systems, which make it easy to transfer inserts between vectors, simplifying the study of multiple aspects of protein function.

A protocol for imaging live or fixed cells expressing HaloTag® protein, for analyzing post-translational modification of labeled fusion proteins, and isolating proteins and protein complexes.