Monitor stem cell pluripotency and differentiation. Amplify stem cell marker transcripts along with a reference transcript in a single tube using StemElite® Primer Pairs and Expression System.
StemElite® Human Pluripotent Transcripts
Convenient 20X primer pairs for NANOG, SOX2, POU5F1, LIN28, KLF4 and MYC markers associated with undifferentiated human embryonic stem cell.
StemElite® Gene Expression System
This novel real-time quantitative PCR (qPCR) system is optimized to amplify a two-color duplex, enabling the user to amplify a transcript of interest as well as a reference gene in a single reaction.
StemElite® Differentiation-Associated Transcripts
Determine whether pluripotential stem cells have differentiated using convenient primer pairs for FOXA2, SOX17 and GATA6, nonspecific differentiation markers for all three embryonic germ layers.
StemElite® Human Heart-Associated Transcripts
Select these primer pairs to identify common mesodermal markers associated with the differentiation of cardiac muscle: NPPA, MYL7, MYL2, MYH6, MYH7, NKX2-5, TNNT2, TNNI3, MEF2C, PLN and GATA4.
A report describing use of the StemElite™ Gene Expression System to assess the expression level of pluripotency-associated transcripts for cells differentiated from human embryonic stem cells.
In this article, we describe use of the GoTaq® qPCR Master Mix with primer pairs that are specific to transcripts of interest to monitor changes in gene expression of treated stem cells.