The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.
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For more information, see the Protocols & Applications Guide.
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S30 T7 High-Yield Protein Expression System
S30 Premix Plus
S30 T7 Control DNA
T7/S30 Extract for Circular DNA
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
All protein coding sequences were cloned into pFN6A and expressed using 1µg of plasmid DNA. Lane 1, no DNA; lane 2, pFN6AhRl; lane 3, pFN6A-HT2; lane 4, pFN6A-β-gal.
L1110, L1115 For Research Use Only. Not for Use in Diagnostic Procedures.