Your choice of E. coli cell-free protein expression systems to translate a variety of input templates and get your results in hours, not days.
S30 T7 High-Yield System
Express up to 500µg/ml protein directly from plasmid DNA in only 1 hour with this E. coli-based system.
T7 S30 System for Circular Templates
To express protein, add plasmid DNA containing a T7 promoter and a ribosomal binding site to the S30 extract. No purification required after expression. Use directly for proteomics related experiments.
S30 Extract System for Linear Templates
Express protein quicky and easily from PCR, DNA fragments, ligated overlapping oligonucleotides, in vitro generated RNA, or prokaryotic RNA.
In this article we discuss factors that improve protein yield with the S30 T7 High-Yield Protein Expression System.