NGF E_max® ImmunoAssay Systems

The NGF E_max® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of biologically active nerve growth factor (NGF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. The system can be used to quantitate NGF in tissue culture supernatants and tissue extracts in the range of 3.9–250pg/ml. Avoid using samples containing high levels of...

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The NGF E_max® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of biologically active nerve growth factor (NGF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. The system can be used to quantitate NGF in tissue culture supernatants and tissue extracts in the range of 3.9–250pg/ml. Avoid using samples containing high levels of IgG such as serum, plasma and spleen.

Features - Benefits

• High Value: Optimized reagents and protocol provided.
• Specificity: Specific detection of NGF; less than 3% cross-reactivity with other related neurotrophic and growth factors.
• Sensitivity: Detect picogram levels of factor per milliliter of sample.
• Flexibility: Available kits provide sufficient reagents for two or five 96-well plates; you can configure your plates as desired.

Plates are not included.

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